Journal: STAR Protocols

Article Title: Protocol for mapping murine myeloid bone marrow progenitors and their differentiation into CD103 + cDC1s and CD301b + cDC2s

doi: 10.1016/j.xpro.2026.104508

Figure Lengend Snippet: From mouse preparation to single cell bone marrow cell suspension (A) Fixation of a euthanized C57BL/6 J mouse to the preparation table. (B) Opening of the abdominal wall. (C and D) Proximal to distal cuts in the direction of the extremities. (E–G) Removal of leg-covering skin and fur. (H) Hip dislocation. (I) Left: Isolation of hindlegs. Bottom: Close-up of the incision. Right: Isolated leg. (J) Left: Feet removal by rotating and pulling. Right: Feet removal by cutting at the ankle joint. (K) Reference picture of connected (left) and disconnected (right) tibia and femur after tissue removal and bone cleaning. (L) Opening of femurs at both ends to allow for flushing out bone marrow. (M) Opening of tibias to allow for flushing out bone marrow. (N) Bone marrow isolation. (O) Reference example of femur (left) and tibia (right). Before flushing (below) and after flushing of bone marrow (above). (P) Left: Correct overlay of the cell suspension on top of Lympholyte-M, without mixing. Right: Lymphocytes collected at the interface after gradient centrifugation.

Article Snippet: C57BL/6 J wildtype mice (female, between 8-15 weeks of age) , Charles River , #C57/BL6′′J′′.

Techniques: Single Cell, Suspension, Isolation, Gradient Centrifugation

Journal: STAR Protocols

Article Title: Protocol for mapping murine myeloid bone marrow progenitors and their differentiation into CD103 + cDC1s and CD301b + cDC2s

doi: 10.1016/j.xpro.2026.104508

Figure Lengend Snippet: Identification of myeloid progenitors in murine bone marrow (A) Annotation of myeloid progenitors in bone marrow single cell suspensions of C57BL/6 J mice via flow cytometry. The provided annotation approach is based on the combined work of Liu et al. and Schlitzer et al. (B) Dimensionality reduction via Uniform Manifold Approximation and Projection (UMAP) of 30,000 cells from CD45+DAPI-LIN- cells. Gates in (A) were mapped onto the UMAP and color coded (left) while visualizing the expression of population defining markers as expression heat maps (right). Unbiased analysis was performed using the FlowJo plugins downsample and UMAP. LIN=lineage; UMAP=uniform manifold approximation and projection; CMP=common myeloid progenitor; GMP=granulocyte-macrophage progenitor; GP=granulocyte progenitor; cMoP=common monocyte progenitor; M.=monocytes; MDP=monocyte-dendritic cell progenitor; CDP=common dendritic cell progenitor.

Article Snippet: C57BL/6 J wildtype mice (female, between 8-15 weeks of age) , Charles River , #C57/BL6′′J′′.

Techniques: Single Cell, Flow Cytometry, Expressing